Resource Library

Product Information

What's New?

Updates on New Resources Available for scM Products. * Please bookmark this link to keep yourself updated on new scM resources uploaded to the libr...

BD Rhapsody™ Analysis Pipeline Updates

v1.9.1 of the BD Rhapsody™ Analysis Pipeline for improved WTA Analysis Released: Improved putative cell calling algorithm to reduce overcalling o...

Technical Bulletins

Technology Bulletins Released for Rhapsody Products: Ordering Additional Indexes for Rhapsody Index primers are used to uniquely label individual...

Brochures

BD™ Rhapsody™ Single-Cell Analysis System A robust, high-throughput microwell based single-cell partitioning system for reliability in single-cel...

Data Sheets

Streamlined Transfer of High-parameter Flow Cytometry Panel from Cell Analyzer to Cell Sorter Prior to In-Depth Single-Cell Multiomic Analysis of ...

Single Cell Multiomics Presentations

CLL phenotyping with BD Rhapsody™ Targeted Panels and BD® AbSeq Assays Single cell multiomics analysis for deep characterization of exhausted T ce...

Rhapsody Instruments and Reagents

Safety Data Sheets

Please follow the following steps to download Safety Data Sheets (SDS) specific to your country. Navigate to BD REGULATORY DOCUMENTS WEBSITE. From...

Instrument Guides

BD Rhapsody Express Instrument and BD Rhapsody Scanner Site Preparation Guide Read this guide prior to installation of the system to ensure that ...

Reagent Specifications

Capturing Single Cell mRNA with the BD Rhapsody™ Single-Cell Analysis System Ultra Quick Reference This ultra-quick reference is intended for exp...

AbSeq

AbSeq Protocols

Cell Labeling Single Cell Labelling with BD™ AbSeq Ab-Oligos This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides). D...

Bioinformatics Guides

BD Single Cell Genomics Bioinformatics Handbook This handbook is a comprehensive reference to help you prepare and analyze single cell libraries ...

AbSeq Reference files

Full list of  BD Ab-Oligo sequences Use online BD AbSeq reference generator to generate AbSeq reference file

Targeted Gene Expression Analysis

Targeted mRNA Protocols

Cell Preparation and Cell Labeling Preparing Single Cell Suspensions This protocol describes preparation of single cell suspensions of commonly u...

Bioinformatics Guides

BD Single Cell Genomics Bioinformatics Handbook This handbook is a comprehensive reference to help you prepare and analyze single cell libraries ...

Pre-designed Panel Gene List

BD Rhapsody™ Immune ResponseTargeted Panel (Human) gene list List of all 399 genes in the Human Immune Response panel with their transcripts and ...

Reference files (.fasta)

1. BD Rhapsody™ Immune Response Panel Hs 2. BD Rhapsody™ Immune Response Panel Mm 3. BD Rhapsody™ T Cell Expression Panel Hs 4. BD Rhapsody™ Onco-B...

Whole Transcriptome Analysis (WTA)

WTA Protocols

Cell Preparation and Cell Labeling Preparing Single Cell Suspensions This protocol describes preparation of single cell suspensions of commonly u...

Bioinformatics Guides

BD Single Cell Genomics Bioinformatics Handbook This handbook is a comprehensive reference to help you prepare and analyze single cell libraries ...

WTA Presentation Slide Decks

BD Rhapsody scM WTA Assay Performance Information

VDJ

VDJ CDR3 Protocols

The following protocols provide instructions on creating single-cell mRNA libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rha...

Bioinformatics Guides

BD Single Cell Genomics Bioinformatics Handbook This handbook is a comprehensive reference to help you prepare and analyze single cell libraries ...

VDJ Presentation Slide Decks

BD Rhapsody VDJ CDR3 Protocol Overview Presentation Slide Deck.

Demo Datasets

Rhapsody VDJ Demo Datasets

VDJ Demo Data The sample used for this demo data is freshly thawed frozen PBMCs. ~3000 cells were captured using BD Rhapsody Single-Cell Analysis S...

Rhapsody WTA Demo Datasets

WTA + Sample Multiplexing This sample is comprised of human cell lines, Jurkat and Ramos, that were labeled with four unique Sample Tags from the B...

Rhapsody Targeted mRNA Demo Datasets

All samples were run with cryopreserved donor-isolated human PBMCs using the pre-designed BD Rhapsody Immune Response Targeted Panel assay.  Human ...

Sample Multiplexing Kit

Protocols

Cell Labeling: 1. Single Cell Labeling with the BD™ Single-Cell Multiplexing Kits: The BD™ Human Single-Cell Multiplexing Kit (Cat. No. 633781) and...