All samples were run with cryopreserved donor-isolated human PBMCs using the pre-designed BD Rhapsody™ Immune Response Panel HS assay.
Human Immune Response
- RSEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on RSEC error correction
- DBEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on DBEC error correction
- Expression_Data.st - Data table of gene counts in sparse format
- Metrics_Summary.csv - Report containing sequencing, molecules, and cell metrics
Human Immune Response + AbSeq
- RSEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on RSEC error correction
- DBEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on DBEC error correction
- Expression_Data.st - Data table of AbSeq and gene counts in sparse format
- Metrics_Summary.csv - Report containing sequencing, molecules, and cell metrics
BD Rhapsody™ Immune Response Panel HS + BD® Single-Cell Multiplexing Kit
- RSEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on RSEC error correction
- DBEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on DBEC error correction
- Expression_Data.st - Data table of gene counts in sparse format
- Metrics_Summary.csv - Report containing sequencing, molecules, and cell metrics
- Sample_Tag_Metrics.csv - Metrics of the Sample Tag reads
- Sample_Tag_calls.csv - Assigned Sample Tag for each putative cell
- SampleTag#_hs_PBMCDonor1.zip - Data tables, expression matrix, and clustering analysis files for Sample Tag #