Targeted Gene Expression Analysis Protocols

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Single Cell Preparation and Cell Labeling

• Preparing Single Cell Suspensions
  • This protocol describes the preparation of single-cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
  • Doc ID: 23-24126(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® Single-Cell Multiplexing Kits
  • The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD® Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD® Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system.
  • Doc ID: 23-21340(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® AbSeq Ab-Oligos (1 to 40 plex)
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining up to 40 antibodies at a time.
  • Doc ID: 23-24262(01)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® AbSeq Ab-Oligos (41 to 100 plex) 
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining 41 - 100 antibodies at a time.
  • Doc ID: 23-22314(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 to 40 plex)
  • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with BD® Single-Cell Multiplexing Kits.
  • Doc ID: 23-21339(02)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (41 to 100 plex)
  • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with BD® Single-Cell Multiplexing Kits.
  • Doc ID: 23-22354(02)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits
  • This protocol describes the use of the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
  • Doc ID: 23-24311(01)
  • Revision date: 12/2022
• Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 plex to 100 plex)
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
  • Doc ID: 23-24312(01)
  • Revision date: 12/2022

Single Cell Capture and cDNA Synthesis

• Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

Sequencing Library Preparation

For use with Enhanced Cartridge Reagent Kit (catalog number 664887)

• BD Rhapsody™ System mRNA Targeted Library Preparation Protocol
  • This protocol provides instructions on creating a single cell mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
  • Doc ID: 23-24121(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Targeted and Sample Tag Library Preparation Protocol
  • This protocol provides instructions on creating single cell mRNA and Sample Tag libraries with the BD RhapsodyTM Single-Cell Analysis system or the BD RhapsodyTM Express Single-Cell Analysis system for sequencing on Illumina sequencers.
  • Doc ID: 23-24122(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Targeted and BD® AbSeq Library Preparation Protocol
  • This protocol provides instructions on creating mRNA and BD® AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
  • Doc ID: 23-24123(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Targeted, Sample Tag, and BD® AbSeq Library Preparation Protocol
  • This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD® AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers. 
  • Doc ID: 23-24124(02)
  • Revision date: 11/2022

 

 

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