BD Rhapsody™ WTA Protocols

An upgraded WTA assay is available now. Visit BD Biosciences website for more information about the NEW BD OMICS-One™ WTA Next Assay

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kits section

Single Cell Capture and cDNA Synthesis Protocols

Single Lane Cartridge on BD Rhapsody™ Express Systems

• Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

8-Lane Cartridge on BD Rhapsody™ HT Xpress Systems

• With Scanner : BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
    • Doc ID: 23-24252(03)
    • Revision date: 04/2025
• Without Scanner : BD Rhapsody™ HT Xpress System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
    • Doc ID: 23-24253(03)
    • Revision date: 04/2025

Sequencing Library Preparation Protocols

• Whole Transcriptome Analysis (WTA) Library Preparation Protocol
  • Doc ID: 23-24117(03)
  • Revision date: 01/2025
• Whole Transcriptome Analysis (WTA) and AbSeq Library Preparation Protocol
  • Doc ID: 23-24118(03)
  • Revision date: 01/2025
• Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
  • Doc ID: 23-24119(03)
  • Revision date: 01/2025
• Whole Transcriptome Analysis (WTA), AbSeq, and Sample Tag Library Preparation Protocol
  • Doc ID: 23-24120(03)
  • Revision date: 01/2025

Mitochondrial Depletion with Jumpcode Genomics kits

• This protocol provides guidelines on how to use the Deplete X kit from Jumpcode Genomics alongside the BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol for removing any undesired mitochondrial transcripts from the WTA library before sequencing.

For data analysis, please see the Bioinformatics Section

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