For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section
For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kits section
Single Cell Capture and cDNA Synthesis Protocols
Single lane cartridge on BD Rhapsody™ Express Single-Cell Analysis system
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Without scanner: Extended-Lysis Single-Cell Capture and cDNA Synthesis with BD Rhapsody™ Express Single-Cell Analysis System Protocol
- Doc ID: 23-24981(01)
- Revision date: 09/2025
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With scanner: Extended-Lysis Single-Cell Capture and cDNA Synthesis with BD Rhapsody™ Single-Cell Analysis System Protocol
- Doc ID: 23-24982(01)
- Revision date: 09/2025
8-lane cartridge on BD Rhapsody™ HT Single-Cell Analysis System
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Without Scanner: Extended-Lysis Single-Cell Capture and cDNA Synthesis with BD Rhapsody™ HT Xpress System Protocol
- Doc ID: 23-24983(01)
- Revision date: 09/2025
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With Scanner: Extended-Lysis Single-Cell Capture and cDNA Synthesis with BD Rhapsody™ HT Single-Cell Analysis System Protocol
- Doc ID: 23-24984(01)
- Revision date: 09/2025
Sequencing Library Preparation Protocols
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BD OMICS-One™ WTA Next Library Preparation Protocol
- Doc ID: 23-24991(01)
- Revision date: 10/2025
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BD OMICS-One™ WTA Next and BD® AbSeq Library Preparation Protocol
- Doc ID: 23-24992(01)
- Revision date: 10/2025
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BD OMICS-One™ WTA Next and Sample Tag Library Preparation Protocol
- Doc ID: 23-24993(01)
- Revision date: 10/2025
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BD OMICS-One™ WTA Next, BD® AbSeq, and Sample Tag Library Preparation Protocol
- Doc ID: 23-24994(01)
- Revision date: 10/2025
Mitochondrial Depletion with Jumpcode Genomics kits
- This protocol provides guidelines on how to use the Deplete X kit from Jumpcode Genomics alongside the BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol for removing any undesired mitochondrial transcripts from the WTA library before sequencing.