Rhapsody WTA Demo Datasets(Enhanced Beads)


WTA

This WTA dataset was generated from 20,000 resting PBMC. Sequencing library was generated using the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit – 4 pack

WTA + AbSeq

This WTA+AbSeq dataset was generated from 10,000 resting PBMC. Cells were labeled with BD® AbSeq Immune Discovery Panel. Sequencing libraries for RNA and AbSeq antibodies were generated using the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit – 4 pack

WTA + AbSeq + SMK

This WTA+AbSeq+SMK dataset was generated from mixed human cell line sample comprised of 5,000 cells that were labeled with three unique Sample Tags from the BD™ Hu Single Cell Sample Multiplexing Kit (Jurkat: ST1; Ramos: ST2; THP-1: ST3). The three labeled samples were then mixed at an equal ratio (1: 1: 1) and labeled with BD® AbSeq Immune Discovery Panel. Sequencing libraries for RNA, AbSeq antibodies, and Sample Tags were generated using the BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit – 4 pack