TCR/BCR Next is our new generation VDJ Full Length assay released in 2024.
You can pair it with WTA(legacy) or WTA Next assay which was released in late 2025.
The following protocols provide instructions on creating single-cell mRNA libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells. To create the library, cDNA of gene targets are encoded on BD Rhapsody™ Cell Capture Beads. This targeted library approach employs a two-step nested amplification, followed by an index PCR step. The generated library can be sequenced on various Illumina sequencers.
BD Rhapsody VDJ protocol is compatible with BD Rhapsody - Targeted, Abseq and SMK protocols either individually or combined.