VDJ full length (TCR/BCR) assay protocols - Mouse

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For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

Single lane cartridge

• Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

8 lane High Throughput cartridge

• With scanner : BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
  • Doc ID: 23-24252(01)
  • Revision date: 03/2023
• Without scanner : BD Rhapsody™ HT Xpress System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
  • Doc ID: 23-24253(01)
  • Revision date: 03/2023

Sequencing Library Preparation

  For use with Enhanced Cartridge Reagent Kit (catalog number 664887), do not use with any other bead versions.

VDJ + WTA (mouse)

1. BD Rhapsody™ System Mouse TCR/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
   
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
   • Doc ID: 23-24364(01)
   • Revision date: 12/2022
2. BD Rhapsody™ System Mouse TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and Sample Tag Library Preparation Protocol
   
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24365(01)
   • Revision date: 12/2022
3. BD Rhapsody™ System Mouse TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and BD® AbSeq Library Preparation Protocol
   
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
   • Doc ID: 23-24366(01)
   • Revision date: 12/2022
4. BD Rhapsody™ System Mouse TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag Library Preparation Protocol
   
   • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24367(01)
   • Revision date: 12/2022

VDJ + Targeted mRNA (mouse)

1. BD Rhapsody™ System Mouse TCR/BCR Full Length and Targeted mRNA Library Preparation Protocol
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
   • Doc ID: 23-24360(01)
   • Revision date: 12/2022
2. BD Rhapsody™ System Mouse TCR/BCR Full Length, Targeted mRNA, and Sample Tag Library Preparation Protocol
   
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24361(01)
   • Revision date: 12/2022
3. BD Rhapsody™ System Mouse TCR/BCR Full Length, Targeted mRNA, and BD® AbSeq Library Preparation Protocol
   • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
   • Doc ID: 23-24362(01)
   • Revision date: 12/2022
4. BD Rhapsody™ System Mouse TCR/BCR Full Length, Targeted mRNA, BD® AbSeq, and Sample Tag Library Preparation Protocol
   • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a Sample Tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24363(01)
   • Revision date: 12/2022

For Data Analysis, please see the Bioinformatics Section

 

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