VDJ full length (TCR/BCR) assay protocols - Human

---

Single Cell Preparation and Cell Labeling

• Preparing Single Cell Suspensions
  • This protocol describes the preparation of single-cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
  • Doc ID: 23-24126(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® Single-Cell Multiplexing Kits
  • The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD® Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD® Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system.
  • Doc ID: 23-21340(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® AbSeq Ab-Oligos (1 to 40 plex)
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining up to 40 antibodies at a time.
  • Doc ID: 23-24262(01)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® AbSeq Ab-Oligos (41 to 100 plex) 
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining 41 - 100 antibodies at a time.
  • Doc ID: 23-22314(02)
  • Revision date: 11/2022
• Single Cell Labelling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 to 40 plex)
  • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with BD® Single-Cell Multiplexing Kits.
  • Doc ID: 23-21339(02)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (41 to 100 plex)
  • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with BD® Single-Cell Multiplexing Kits.
  • Doc ID: 23-22354(02)
  • Revision date: 11/2022
• Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits
  • This protocol describes the use of the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
  • Doc ID: 23-24311(01)
  • Revision date: 12/2022
• Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 plex to 100 plex)
  • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
  • Doc ID: 23-24312(01)
  • Revision date: 12/2022

Single Cell Capture and cDNA Synthesis

• Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

Sequencing Library Preparation

For use with Enhanced Cartridge Reagent Kit (catalog number 664887)

VDJ + WTA (human)

• BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)
  
  • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
  • Doc ID: 23-24017(03)
  • Revision date: 11/2022
• BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and Sample Tag
  
  • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
  • Doc ID: 23-24018(03)
  • Revision date: 11/2022
• BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and BD® AbSeq
  
  • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
  • Doc ID: 23-24019(03)
  • Revision date: 11/2022
• BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag
  
  • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
  • Doc ID: 23-24020(03)
  • Revision date: 11/2022

VDJ + Targeted mRNA (human)

1. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and Targeted mRNA
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
   • Doc ID: 23-24013(03)
   • Revision date: 11/2022
2. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and Sample Tag
   
   • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24014(03)
   • Revision date: 11/2022
3. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and BD® AbSeq
   • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
   • Doc ID: 23-24015(03)
   • Revision date: 11/2022
4. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, BD® AbSeq, and Sample Tag
   • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
   • Doc ID: 23-24016(03)
   • Revision date: 11/2022

 

 

#23