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VDJ full length (TCR/BCR) assay protocols - Human
Single Cell Preparation and Cell Labeling
- Preparing Single Cell Suspensions
- This protocol describes the preparation of single-cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
- Doc ID: 23-24126(02)
- Revision date: 11/2022
- Single Cell Labelling with BD® Single-Cell Multiplexing Kits
- The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD® Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD® Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system.
- Doc ID: 23-21340(02)
- Revision date: 11/2022
- Single Cell Labelling with BD® AbSeq Ab-Oligos (1 to 40 plex)
- This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining up to 40 antibodies at a time.
- Doc ID: 23-24262(01)
- Revision date: 11/2022
- Single-Cell Labeling with BD® AbSeq Ab-Oligos (41 to 100 plex)
- This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining 41 - 100 antibodies at a time.
- Doc ID: 23-22314(02)
- Revision date: 11/2022
- Single Cell Labelling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 to 40 plex)
- This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with BD® Single-Cell Multiplexing Kits.
- Doc ID: 23-21339(02)
- Revision date: 11/2022
- Single-Cell Labeling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (41 to 100 plex)
- This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with BD® Single-Cell Multiplexing Kits.
- Doc ID: 23-22354(02)
- Revision date: 11/2022
- Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits
- This protocol describes the use of the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
- Doc ID: 23-24311(01)
- Revision date: 12/2022
- Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 plex to 100 plex)
- This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) with the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852).
- Doc ID: 23-24312(01)
- Revision date: 12/2022
Single Cell Capture and cDNA Synthesis
- Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
- This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
- Doc ID: 23-22952(02)
- Revision date: 11/2022
- Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
- This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
- Doc ID: 23-22951(02)
- Revision date: 11/2022
Sequencing Library Preparation
For use with Enhanced Cartridge Reagent Kit (catalog number 664887)
VDJ + WTA (human)
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)
- This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
- Doc ID: 23-24017(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and Sample Tag
- This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
- Doc ID: 23-24018(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), and BD® AbSeq
- This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
- Doc ID: 23-24019(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag
- This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
- Doc ID: 23-24020(03)
- Revision date: 11/2022
VDJ + Targeted mRNA (human)
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and Targeted mRNA
- This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
- Doc ID: 23-24013(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and Sample Tag
- This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
- Doc ID: 23-24014(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and BD® AbSeq
- This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
- Doc ID: 23-24015(03)
- Revision date: 11/2022
- BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, BD® AbSeq, and Sample Tag
- This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
- Doc ID: 23-24016(03)
- Revision date: 11/2022
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