WTA Protocols

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

Single lane cartridge

• Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

8 lane High Throughput cartridge

• With scanner : BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
  • Doc ID: 23-24252(01)
  • Revision date: 03/2023
• Without scanner : BD Rhapsody™ HT Xpress System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
  • Doc ID: 23-24253(01)
  • Revision date: 03/2023

Sequencing Library Preparation Protocols

  For use with Enhanced Cartridge Reagent Kit (catalog number 664887), do not use with v1 beads. 

• BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
  • This protocol provides instructions on creating a single cell whole transcriptome mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina® sequencers.
  • Doc ID: 23-24117(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and AbSeq Library Preparation Protocol
  • This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein-detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. 
  • Doc ID: 23-24118(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
  • This protocol provides instructions on creating single cell whole transcriptome mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers.
  • Doc ID: 23-24119(02)
  • Revision date: 11/2022
• BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA), AbSeq, and Sample Tag Library Preparation Protocol
  • This protocol provides instructions on creating single-cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers.
  • Doc ID: 23-24120(02)
  • Revision date: 11/2022

Mitochondrial Depletion with Jumpcode Genomics kits

• This protocol provides guidelines on how to use the Deplete X kit from Jumpcode Genomics alongside the BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol for removing any undesired mitochondrial transcripts from the WTA library before sequencing.

For Data Analysis, please see the Bioinformatics Section

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