VDJ full length (TCR/BCR) assay protocols - Human


Single Cell Preparation and Cell Labeling

Single Cell Capture and cDNA Synthesis

Sequencing Library Preparation

For use with Enhanced Cartridge Reagent Kit (catalog number 664887)

VDJ + WTA (human)

VDJ + Targeted mRNA (human)

  1. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and Targeted mRNA
    • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
    • Doc ID: 23-24013(03)
    • Revision date: 11/2022
  2. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and Sample Tag
    • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
    • Doc ID: 23-24014(03)
    • Revision date: 11/2022
  3. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and BD® AbSeq
    • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
    • Doc ID: 23-24015(03)
    • Revision date: 11/2022
  4. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, BD® AbSeq, and Sample Tag
    • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
    • Doc ID: 23-24016(03)
    • Revision date: 11/2022

 

 

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