Articles in this section

VDJ full length (TCR/BCR) assay protocols - Human

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

Single lane cartridge

8 lane High Throughput cartridge

  •  

Sequencing Library Preparation

caution.png  For use with Enhanced Cartridge Reagent Kit (catalog number 664887), do not use with any other bead versions.

VDJ + WTA (human)

VDJ + Targeted mRNA (human)

  1. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and Targeted mRNA
    • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries.
    • Doc ID: 23-24013(03)
    • Revision date: 11/2022
  2. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and Sample Tag
    • This protocol enables high-throughput single-cell transcriptome analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
    • Doc ID: 23-24014(03)
    • Revision date: 11/2022
  3. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, and BD® AbSeq
    • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries.
    • Doc ID: 23-24015(03)
    • Revision date: 11/2022
  4. BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length, Targeted mRNA, BD® AbSeq, and Sample Tag
    • This protocol enables high-throughput single-cell transcriptome and protein analysis alongside TCR and BCR profiling of individual cells captured on the BD Rhapsody™ system, providing instructions for amplifying Illumina-compatible single-cell barcoded mRNA, AbSeq, TCR, and BCR libraries. The addition of a sample-tag library enables demultiplexing of samples that were pooled before capture on the BD Rhapsody™ system.
    • Doc ID: 23-24016(03)
    • Revision date: 11/2022

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Data Analysis, please see the Bioinformatics Section

 

#23

Was this article helpful?
0 out of 0 found this helpful