In this section
Articles in this section
BD Rhapsody™ Analysis Pipeline Updates
- v1.9.1 of the BD Rhapsody™ Analysis Pipeline for improved WTA Analysis Released:
- Improved putative cell calling algorithm to reduce overcalling of putative cells in high cell input experiments
- Updated alignment settings to improve AbSeq mapping when R2 read length is greater than 75 bases
- v1.9 BD Rhapsody™ Targeted Analysis Pipeline and BD Rhapsody™ WTA Analysis Pipeline Released:
- Improved FASTQ file pairing - filenames are flexible and pairing is now based on read sequence identifier
- Optimized pipeline in various steps for memory and storage usage
- Fixed bugs related to Sample Multiplexing Kit noise and DBEC mean molecule metric
- BD Rhapsody™ Targeted Analysis Pipeline:
- Support for BD Rhapsody™ VDJ CDR3 protocol
- Read and molecule counts for targets from same gene symbol are combined in the output tables
- Updated Bowtie2 alignment parameters for improved sensitivity
- BD Rhapsody™ WTA Analysis Pipeline:
- Updated Pct_Cellular Metrics calculations to match Bioinformatics handbook descriptions
- Added support for supplemental reference fasta files, which allow alignment to transgenes, like viral RNA or GFP
- Updated STAR alignment parameters for improved sensitivity