BD Rhapsody™ Analysis Pipeline Updates


  • v1.9.1 of the BD Rhapsody™ Analysis Pipeline for improved WTA Analysis Released:
    • Improved putative cell calling algorithm to reduce overcalling of putative cells in high cell input experiments
    • Updated alignment settings to improve AbSeq mapping when R2 read length is greater than 75 bases
  • v1.9 BD Rhapsody™ Targeted Analysis Pipeline and BD Rhapsody™ WTA Analysis Pipeline Released:
    • Improved FASTQ file pairing - filenames are flexible and pairing is now based on read sequence identifier
    • Optimized pipeline in various steps for memory and storage usage
    • Fixed bugs related to Sample Multiplexing Kit noise and DBEC mean molecule metric
    • BD Rhapsody™ Targeted Analysis Pipeline:
      • Support for BD Rhapsody™ VDJ CDR3 protocol
      • Read and molecule counts for targets from same gene symbol are combined in the output tables
      • Updated Bowtie2 alignment parameters for improved sensitivity
    • BD Rhapsody™ WTA Analysis Pipeline:
      • Updated Pct_Cellular Metrics calculations to match Bioinformatics handbook descriptions
      • Added support for supplemental reference fasta files, which allow alignment to transgenes, like viral RNA or GFP
      • Updated STAR alignment parameters for improved sensitivity