Technical Bulletins

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Technology Bulletins Released for Rhapsody Products:

1. Ordering Additional Indexes for Rhapsody
   • Index primers are used to uniquely label individual libraries so that multiple libraries can be sequenced in a single Illumina® sequencing run. This technical bulletin provides quick guides and instructions for ordering additional indexing primers for BD Rhapsody™ Sequencing Libraries.
   • Doc ID: 23-22747-00
   • Revision date: 06/2020
2. How to Operate the BD Rhapsody™ Targeted Analysis VDJ CDR3 Pipeline
   • This technical bulletin is a supplement to the BD® Single-Cell Multiomics Bioinformatics Handbook and provides a step-by-step instructions on how to operate the BD Rhapsody Targeted Analysis VDJ CDR3 Pipeline.
   • Doc ID: 23-22817-00
   • Revision date: 07/2020
3. Processing nuclei samples on the BD Rhapsody™ Single-Cell Analysis System
   • In this technical bulletin, we show how isolated nuclei can be captured and amplified on the BD Rhapsody™ Single-Cell Analysis System.
   • Doc ID: 23-23161-00
   • Revision date: 10/2020
4. Co-staining cells with fluorescent antibodies and BD Oligo-Conjugated Antibodies
   • Here, we address how to co-stain cells with the same clone of both fluorescent antibodies and oligonucleotide-conjugated antibodies (BD® Single-Cell Multiplexing Kit or BD® AbSeq Ab-Oligos).
   • Doc ID: 23-23247-00
   • Revision date: 03/2021
5. Integration of the Element AVITITM System with BD RhapsodyTM Single-Cell Assays creates a powerful solution for the immuno-oncology researcher
   • In this application note, we show compatibility of Element’s novel sequencing system with BD RhapsodyTM Single-Cell Assays.
   • Doc ID: BD-63787 (v1.0)
   • Revision date: 10/2022
6. Highly scalable and more sensitive CRISPR screens using the BD Rhapsody™ Single-Cell Analysis System