WTA Protocols


Single Cell Preparation and Cell Labeling

  1. Preparing Single Cell Suspensions
    • This protocol describes the preparation of single-cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ Cartridge. This protocol does not describe tissue dissociation.
    • Doc ID: 210964 Rev. 2.0/23-24126(01)
    • Revision date: 11/2021
  2. Single Cell Labelling with BD® Single-Cell Multiplexing Kits
    • The BD® Human Single-Cell Multiplexing Kit (Cat. No. 633781) and the BD® Mouse Immune Single-Cell Multiplexing Kit (Cat. No. 633793) utilize an innovative antibody-oligo technology to provide higher sample throughput for single cell library preparation. Every antibody-oligo in the BD® Human Single-Cell Multiplexing Kit, referred to as a Sample Tag, has a unique sample oligo barcode conjugated to a human universal antibody and every Sample Tag in the BD® Mouse Immune Single-Cell Multiplexing Kit is conjugated to an Anti-Mouse CD45, Clone 30-F11 antibody. Up to 12 samples can be labelled and pooled prior to single cell capture with the BD Rhapsody™ Single-Cell Analysis system.
    • Doc ID: 210970 Rev. 3.0/23-21340(01)
    • Revision date: 11/2021
  3. Single Cell Labelling with BD® AbSeq Ab-Oligos (1 to 40 plex)
    • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining up to 40 antibodies at a time.
    • Doc ID: 214394 Rev. 1.0
    • Revision date: 07/2018
  4. Single-Cell Labeling with BD® AbSeq Ab-Oligos (41 to 100 plex) 
    • This protocol describes the use of BD® AbSeq Ab-Oligos (antibody-oligonucleotides) when staining 41 - 100 antibodies at a time.
    • Doc ID: 23-22314(01)
    • Revision date: 11/2021
  5. Single Cell Labelling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (1 to 40 plex)
    • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with BD® Single-Cell Multiplexing Kits.
    • Doc ID: 214419 Rev. 3.0/23-21339(01)
    • Revision date: 11/2021
  6. Single-Cell Labeling with BD® Single-Cell Multiplexing Kits and BD® AbSeq Ab-Oligos (41 to 100 plex)
    • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with BD® Single-Cell Multiplexing Kits.
    • Doc ID: 23-22354(01)
    • Revision date: 11/2021

Single Cell Capture and cDNA Synthesis

  1. Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
    • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
    • Doc ID: 210967 Rev. 3.0/23-22952(01)
    • Revision date: 01/2022
  2. Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
    • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
    • Doc ID: 210966 Rev. 3.0/23-22951(01)
    • Revision date: 01/2022

Sequencing Library Preparation

For use with Enhanced Cartridge Reagent Kit (catalog number 664887)

  1. BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
    • This protocol provides instructions on creating a single cell whole transcriptome mRNA library after cell capture on the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina® sequencers.
    • Doc ID: 23-24117(01)
    • Revision date: 12/2021
  2. BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and AbSeq Library Preparation Protocol
    • This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein-detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. 
    • Doc ID: 23-24118(01)
    • Revision date: 12/2021
  3. BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) and Sample Tag Library Preparation Protocol
    • This protocol provides instructions on creating single cell whole transcriptome mRNA and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers.
    • Doc ID: 23-24119(01)
    • Revision date: 12/2021
  4. BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA), AbSeq, and Sample Tag Library Preparation Protocol
    • This protocol provides instructions on creating single-cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers.
    • Doc ID: 23-24120(01)
    • Revision date: 12/2021

 

 

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