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WTA Protocols

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

Single lane cartridge

8 lane High Throughput cartridge

Sequencing Library Preparation Protocols

caution.png  The protocols below are meant for use with the Enhanced Cartridge Reagent Kit (catalog number 664887 or 667052), do not use these protocols with the v1 beads or any older bead versions.

Mitochondrial Depletion with Jumpcode Genomics kits

  • This protocol provides guidelines on how to use the Deplete X kit from Jumpcode Genomics alongside the BD Rhapsody™ System mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol for removing any undesired mitochondrial transcripts from the WTA library before sequencing.

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Data Analysis, please see the Bioinformatics Section

 

 

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