WTA + Sample Multiplexing
This sample is comprised of human cell lines, Jurkat and Ramos, that were labeled with four unique Sample Tags from the BD® Single-Cell Multiplexing Kit Hu (Jurkat: ST3, ST5; Ramos: ST7, ST9). The four labeled samples were then mixed at an equal ratio (1: 1: 1: 1) and processed using the BD Rhapsody™ Single-Cell Analysis System. Sequencing libraries were generated using the BD Rhapsody™ WTA Amplification kit.
The zip file contains the following:
- RSEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on RSEC error correction
- Expression_Data.st - Data table of gene counts in sparse format
- Metrics_Summary.csv - Report containing sequencing, molecules, and cell metrics
- Sample_Tag_Metrics.csv - Metrics of the Sample Tag reads
- Sample_Tag_calls.csv - Assigned Sample Tag for each putative cell
- SampleTag#_hs.zip - Data tables, expression matrix, and clustering analysis files for each Sample Tag
WTA + Sample Multiplexing + AbSeq
Freshly isolated human PBMCs were grown for 24 hours in the presence of CD3/CD28 stimulation ("activated") and compared to PBMCs grown for 24 hours in regular media ("naive"). Cells were then labeled with a 20plex panel of BD® AbSeq Antibody-Oligos consisting of lineage markers and T-cell activation markers, as well as unique Sample Tags from the BD® Single-Cell Multiplexing Kit (resting PBMC: ST1; activated PBMC: ST2). The cells were mixed at a 1:1 ratio and processed using the BD Rhapsody™ Single-Cell Analysis System. Sequencing libraries for RNA and AbSeq antibodies were generated using the BD Rhapsody™ WTA Amplification kit.
The zip file contains the following:
- RSEC_MolsPerCell.csv - Data table containing molecules per gene per cell based on RSEC error correction
- Expression_Data.st - Data table of gene counts in sparse format
- Metrics_Summary.csv - Report containing sequencing, molecules, and cell metrics
- Sample_Tag_Metrics.csv - Metrics of the Sample Tag reads
- Sample_Tag_calls.csv - Assigned Sample Tag for each putative cell
- SampleTag#_hs.zip - Data tables, expression matrix, and clustering analysis files for each Sample Tag