VDJ full length (TCR/BCR Next) assay protocols - Human

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

Replace beads mentioned in these protocols with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052) for TCR/BCR Next Assays

Single lane cartridge -

• Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

8 lane High Throughput cartridge

• With scanner : BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
  • Doc ID: 23-24252(01)
  • Revision date: 03/2023
• Without scanner : BD Rhapsody™ HT Xpress System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
  • Doc ID: 23-24253(01)
  • Revision date: 03/2023

Sequencing Library Preparation

For use with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052), do not use with any other bead versions

VDJ + Targeted (human)

1. Human TCR/BCR Next and Targeted mRNA Library Preparation Protocol
   • Doc ID : 23-24504(01)
   • Updated : 2024-01
2. Human TCR/BCR Next, Targeted mRNA, and Sample Tag Library Preparation Protocol
   • Doc ID : 23-24505(01)
   • Updated : 2024-01
3. Human TCR/BCR Next, Targeted mRNA, and BD®AbSeq Library Preparation Protocol
   • Doc ID : 23-24506(01)
   • Updated : 2024-01
4. Human TCR/BCR Next, Targeted mRNA, BD®AbSeq, and Sample Tag Library Preparation Protocol
   • Doc ID : 23-24507(01)
   • Updated : 2024-01

VDJ + WTA (human)

1. Human TCR/BCR Next and mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
   • Doc ID : 23-24508(01)
   • Updated : 2024-01
2. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), and BD®AbSeq Library Preparation Protocol
   • Doc ID : 23-24510(01)
   • Updated : 2024-01
3. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), and Sample Tag Library Preparation Protocol
   • Doc ID : 23-24509(01)
   • Updated : 2024-01
4. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag Protocol
   • Doc ID : 23-24511(01)
   • Updated : 2024-01

For Data Analysis, please see the Bioinformatics Section