VDJ full length (TCR/BCR Next) assay protocols - Human


Bulb, idea, idea bulb, light bulb icon - Free downloadFor Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kit (SMK and Flex SMK) section

Single Cell Capture and cDNA Synthesis Protocols

caution.png Replace beads mentioned in these protocols with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052) for TCR/BCR Next Assays

Single lane cartridge -

8 lane High Throughput cartridge

Sequencing Library Preparation

caution.png For use with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052), do not use with any other bead versions

VDJ + Targeted (human)

  1. Human TCR/BCR Next and Targeted mRNA Library Preparation Protocol
    • Doc ID : 23-24504(01)
    • Updated : 06/2024
  2. Human TCR/BCR Next, Targeted mRNA, and Sample Tag Library Preparation Protocol
    • Doc ID : 23-24505(01)
    • Updated : 06/2024
  3. Human TCR/BCR Next, Targeted mRNA, and BD®AbSeq Library Preparation Protocol
    • Doc ID : 23-24506(01)
    • Updated : 06/2024
  4. Human TCR/BCR Next, Targeted mRNA, BD®AbSeq, and Sample Tag Library Preparation Protocol
    • Doc ID : 23-24507(01)
    • Updated : 06/2024

VDJ + WTA (human)

  1. Human TCR/BCR Next and mRNA Whole Transcriptome Analysis (WTA) Library Preparation Protocol
    • Doc ID : 23-24508(01)
    • Updated : 06/2024
  2. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), and BD®AbSeq Library Preparation Protocol
    • Doc ID : 23-24510(01)
    • Updated : 06/2024
  3. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), and Sample Tag Library Preparation Protocol
    • Doc ID : 23-24509(01)
    • Updated : 06/2024
  4. Human TCR/BCR Next, mRNA Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag Protocol
    • Doc ID : 23-24511(01)
    • Updated : 06/2024

Bulb, idea, idea bulb, light bulb icon - Free downloadFor Data Analysis, please see the Bioinformatics Section