TCR/BCR Next Assay (Human) + Targeted Gene Expression/WTA Protocols

An upgraded WTA assay is available now. Visit the BD Biosciences website for more information about the NEW BD OMICS-One™ WTA Next Assay

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section 

For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kits section

Single Cell Capture and cDNA Synthesis Protocols(legacy version)

Single Lane Cartridge on BD Rhapsody™ Express Systems

• Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Express Single-Cell Analysis system.
  • Doc ID: 23-22952(02)
  • Revision date: 11/2022
• With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Single-Cell Analysis System
  • This protocol describes cell loading in the BD Rhapsody™ Cartridge and single-cell capture with the BD Rhapsody™ Single-Cell Analysis System with the BD Rhapsody™ Scanner.
  • Doc ID: 23-22951(02)
  • Revision date: 11/2022

8-Lane Cartridge on BD Rhapsody™ HT Xpress Systems

• With Scanner : BD Rhapsody™ HT Single-Cell Analysis System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Single-Cell Analysis System.
    • Doc ID: 23-24252(03)
    • Revision date: 04/2025
• Without Scanner : BD Rhapsody™ HT Xpress System Single-Cell Capture and cDNA Synthesis
  • This protocol describes cell loading in the BD Rhapsody™ 8-Lane Cartridge and single-cell capture with the BD Rhapsody™ HT Xpress System.
    • Doc ID: 23-24253(03)
    • Revision date: 04/2025

Sequencing Library Preparation Protocols

For use with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052), do not use with any other bead versions

BD Rhapsody™ TCR/BCR Next Assay (Human) + BD Rhapsody™ Targeted Gene Expression Assay

• Human TCR/BCR Next and Targeted mRNA Library Preparation Protocol
  • Doc ID : 23-24504(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Targeted mRNA, and Sample Tag Library Preparation Protocol
  • Doc ID : 23-24505(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Targeted mRNA, and BD®AbSeq Library Preparation Protocol
  • Doc ID : 23-24506(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Targeted mRNA, BD®AbSeq, and Sample Tag Library Preparation Protocol
  • Doc ID : 23-24507(01)
  • Updated : 2024-01

BD Rhapsody™ TCR/BCR Next Assay (Human) + BD Rhapsody™ WTA Assay(legacy version)

• Human TCR/BCR Next and Whole Transcriptome Analysis (WTA) Library Preparation Protocol
  • Doc ID : 23-24508(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), and BD®AbSeq Library Preparation Protocol
  • Doc ID : 23-24510(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), and Sample Tag Library Preparation Protocol
  • Doc ID : 23-24509(01)
  • Updated : 2024-01
• Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag Protocol
  • Doc ID : 23-24511(01)
  • Updated : 2024-01

For Data Analysis, please see the Bioinformatics Section