An upgraded WTA assay is available now. Visit BD Biosciences website for more information about the NEW BD OMICS-One™ WTA Next Assay
For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section
For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Section or the BD® Single-Cell Multiplexing Kits section
Single Cell Capture and cDNA Synthesis Protocols
Single Lane Cartridge on BD Rhapsody™ Express Systems
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Without scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System
- Doc ID: 23-24986(01)
- Revision date: 09/2025
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With scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ Express Single-Cell Analysis System and BD Rhapsody™ Scanner
- Doc ID: 23-24987(01)
- Revision date: 09/2025
8-Lane Cartridge on BD Rhapsody™ HT Xpress Systems
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Without Scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ HT Xpress System
- Doc ID: 23-24988(01)
- Revision date: 09/2025
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With Scanner : Single-Cell Capture and cDNA Synthesis with the BD Rhapsody™ HT Single-Cell Analysis System and BD Rhapsody™ Scanner
- Doc ID: 23-24989(01)
- Revision date: 09/2025
Sequencing Library Preparation Protocols
For use with BD Rhapsody™ Enhanced Cartridge Reagent Kit V3(667052), do not use with any other bead versions
BD Rhapsody™ TCR/BCR Next Assay (Human) + BD Rhapsody™ Targeted Gene Expression Assay
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Human TCR/BCR Next and Targeted mRNA Library Preparation Protocol
- Doc ID : 23-24504(01)
- Updated : 2024-01
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Human TCR/BCR Next, Targeted mRNA, and Sample Tag Library Preparation Protocol
- Doc ID : 23-24505(01)
- Updated : 2024-01
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Human TCR/BCR Next, Targeted mRNA, and BD®AbSeq Library Preparation Protocol
- Doc ID : 23-24506(01)
- Updated : 2024-01
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Human TCR/BCR Next, Targeted mRNA, BD®AbSeq, and Sample Tag Library Preparation Protocol
- Doc ID : 23-24507(01)
- Updated : 2024-01
BD Rhapsody™ TCR/BCR Next Assay (Human) + BD Rhapsody™ WTA Assay
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Human TCR/BCR Next and Whole Transcriptome Analysis (WTA) Library Preparation Protocol
- Doc ID : 23-24508(01)
- Updated : 2024-01
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Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), and BD®AbSeq Library Preparation Protocol
- Doc ID : 23-24510(01)
- Updated : 2024-01
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Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), and Sample Tag Library Preparation Protocol
- Doc ID : 23-24509(01)
- Updated : 2024-01
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Human TCR/BCR Next, Whole Transcriptome Analysis (WTA), BD® AbSeq, and Sample Tag Protocol
- Doc ID : 23-24511(01)
- Updated : 2024-01