Mimosa Sarma

1. User Guides and Protocols
2. ATAC-Seq Epigenomic Profiling

For Single Nuclei Preparation, see Single Nuclei Sample Prep Sequencing-ready Library Preparation Protocols   For use with BD Rhapsody™ HT Enhanced Cell Capture Beads V3 (catalog number 667052),...

• Edited November 20, 2024 23:38
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1. User Guides and Protocols
2. TCR/BCR Profiling (VDJ) Assay

Here, we offer a multiomic approach for profiling T-cells at the single-cell level using two powerful technologies: Immudex® dCODE Dextramer® (RiO) Reagents and the BD Rhapsody™ Single-Cell Analysi...

• Edited December 03, 2024 01:27
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1. User Guides and Protocols
2. TCR/BCR Profiling (VDJ) Assay
3. BD Rhapsody™ TCR/BCR Next Multiomic Assays(Full-Length VDJ Profiling)

Human TCR/BCR Next dataset can be found here This demo data was generated from an experiment using previously frozen human PBMCs, in which ~8,000 cells were captured with the BD Rhapsody™ Single-...

• Edited December 03, 2024 01:26
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1. User Guides and Protocols
2. TCR/BCR Profiling (VDJ) Assay
3. BD Rhapsody™ TCR/BCR Next Multiomic Assays(Full-Length VDJ Profiling)

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section  For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Sec...

• Edited April 28, 2025 21:48
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1. User Guides and Protocols
2. TCR/BCR Profiling (VDJ) Assay
3. BD Rhapsody™ TCR/BCR Next Multiomic Assays(Full-Length VDJ Profiling)

For Single Cell Preparation and Preservation Protocols, see Sample Prep and Preservation Section  For Cell Labelling Protocols, see BD® AbSeq Intracellular and Cell Surface Protein Profiling Sec...

• Edited May 20, 2025 17:33
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1. User Guides and Protocols
2. Sample Prep and Preservation

For WTA : Processing nuclei samples on the BD Rhapsody™ Single-Cell Analysis System In this technical bulletin, we show how isolated nuclei can be captured and amplified on the BD Rhapsody™ Sing...

• Edited February 03, 2025 18:04
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1. User Guides and Protocols
2. BD® Single-Cell Multiplexing Kit (SMK and Flex SMK)

Single-Cell Labeling with BD® Flex Single-Cell Multiplexing Kits This protocol describes the use of the BD® Flex Single-Cell Multiplexing Kits (Cat. no. 633849-633852). Doc ID: 23-24311(01) Revi...

• Edited August 30, 2024 23:20
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1. User Guides and Protocols
2. BD Rhapsody™ System Instruments
3. BD Rhapsody™ Scanner

BD Rhapsody™ Express Instrument and BD Rhapsody™ Scanner Site Preparation Guide Read this guide prior to installation of the system to ensure that all site requirements are met. Provides: Dimen...

• Edited December 03, 2024 00:44
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1. User Guides and Protocols
2. Sample Prep and Preservation

Preparing Single Cell Suspensions This protocol describes the preparation of single-cell suspensions of commonly used immune cell types. A single cell suspension can be loaded into a BD Rhapsody™ ...

• Edited June 06, 2024 00:44
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1. User Guides and Protocols
2. BD® AbSeq Intracellular and Cell Surface Protein Profiling(CITE-Seq)

Intracellular CITE-seq with 24-hr Preservation Demo Data This demo data was generated from an intracellular CITE-seq experiment on BD Rhapsody™ System multiplexing resting and PMA & Ionomycin stimu...

• Edited September 05, 2024 19:04
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