AbSeq Protocols


Cell Labeling

  1. Single Cell Labelling with BD™ AbSeq Ab-Oligos
    • This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides).
    • Doc ID: 214394 Rev. 1.0
    • Revision date: 07/2018
  2. Single Cell Labelling with the BD Single-Cell Multiplexing Kit and BD® AbSeq Ab-Oligos (1 plex to 40 plex)
    • This protocol describes use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(1 plex to 40 plex) with the BD® Single-Cell Multiplexing Kit.
    • Doc ID: 214419 Rev. 2.0
    • Revision date: 02/2019
  3. Single-Cell Labeling with BD Single-Cell Multiplexing Kit and BD® AbSeq Ab-Oligos (41 plex to 100 plex)
    • This protocol describes the use of BD AbSeq Ab-Oligos (antibody-oligonucleotides)(41 plex to 100 plex) with the BD® Human Single-Cell Multiplexing Kit.
    • Doc ID: 23-22354-00
    • Revision date: 05/2020

Sequencing Library Preparation

  1. mRNA Targeted and BD™ AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit
    • This protocol provides instructions on creating mRNA and BD™ AbSeq single cell libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
    • Doc ID: 214293 Rev.3.0/23-21343-00
    • Revision date: 02/2019
  2. mRNA Targeted, Sample Tag, and BD™ AbSeq Library Preparation with the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit
    • This protocol provides instructions on creating single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system for sequencing on Illumina sequencers.
    • Doc ID: 214508 Rev.3.0/23-21335-00
    • Revision date: 02/2019
  3. mRNA Whole Transcriptome Analysis (WTA) and AbSeq Library Preparation Protocol
    • This protocol provides instructions on creating single cell whole transcriptome mRNA and AbSeq protein detection libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers. 
    • Doc ID: 23-21751-00
    • Revision date: 07/2019
  4. mRNA Whole Transcriptome Analysis (WTA), AbSeq, and Sample Tag Library Preparation Protocol
    • This protocol provides instructions on creating single cell whole transcriptome mRNA, AbSeq, and Sample Tag libraries after cell capture on the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina sequencers. 
    • Doc ID: 23-21752-00
    • Revision date: 07/2019
  5. VDJ CDR3 and BD AbSeq Library Preparation Protocol  (Human)
    • This protocol provides instructions on creating single-cell mRNA and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells. 
    • Doc ID: 23-22203-01
    • Revision date: 05/2020
  6. VDJ CDR3, Sample Tag, and BD AbSeq Library Preparation Protocol (Human)
    • This protocol provides instructions on creating single-cell mRNA, Sample Tag, and BD Abseq libraries with the BD Rhapsody™ Single-Cell Analysis System or the BD Rhapsody™ Express Single-Cell Analysis System for sequencing on Illumina® sequencers. It is also intended to provide a method to enable a high-throughput detection of VDJ recombination events by generating sequencer-ready libraries specific to the CDR3-specific antigen binding regions of B and T cells for human samples. 
    • Doc ID: 23-22204-01
    • Revision date: 05/2020

 

 

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