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Whole Transcriptome Analysis (WTA)

The BD Rhapsody™ Whole Transcriptome Analysis (WTA) Assay enables unbiased 3'-based capture, amplification and detection of transcriptomes at the single-cell level.

Start here for a filtered table containing only WTA FAQ from the master document. Easily searchable with CMD+F on a Mac or CTRL+F on a PC.

If I am working with a species other than mouse or human, where can I download the reference file?

You can download reference FASTA and GTF reference files from Ensembl or NCBI. Please make sure to generate STAR indexed reference before running the pipeline.

 

Can I detect gene variants using the BD Rhapsody™ WTA or targeted assays?

It is difficult to detect gene variants using WTA or targeted assays. The BD Rhapsody™ WTA and targeted assays use 3'-end capture technology and sequence a short fragment on the 3'-end of the transcript, which is mostly a non-coding region.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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