Experimental Workflow How-to videos

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BD Rhapsody™ Single-Cell Analysis System - Flow Co-Staining

Prior to capturing cells on the BD Rhapsody™ Single-cell Analysis System, cell sorting may be required to enrich specific cellular phenotypes. In this tutorial, we will walk you through our recommendations for how to co-stain Oligo-conjugated AbSeq™ and/or Sample Tag antibodies with Fluorochrome conjugated antibodies that share the same clone. In doing so, you can save time and sample with one antibody panel for your entire workflow from flow sorting to single-cell capture.

Labeling with BD® AbSeq and BD® Single-Cell Multiplexing Kit

How to co-label single cells with Ab-Oligos and/or SMK prior to single cell multiomics analysis.

Counting Cells with the BD Rhapsody™ Single Cell Analysis System

In this video, we examine the techniques you’ll use to count cells using the BD Rhapsody™ Scanner.

Capturing Single Cells with the BD Rhapsody™ Express Single-Cell Analysis System

In this video, we will demonstrate how to capture single cells using the BD Rhapsody™ Express Single-Cell Analysis System. Please note that now we have a higher throughput 8-lane cartridge available.

BD Rhapsody™ Single-Cell Analysis System - Cartridge Workflow

The BD Rhapsody™ Scanner reports quality metrics in real-time at each step of your single cell assay from initial cell capture to bead retrieval. In this video, we will demonstrate how to use the BD Rhapsody™ Scanner to check the quality of your single cell multiomics experiments.

Post PCR1 Purification for BD Rhapsody scM Library Preparation

In this video, we will demonstrate how to purify PCR1 products by performing double-sided size selection using AMPure beads as a part of the BD Rhapsody Library Preparation

BD Rhapsody™ Single-Cell Analysis System - BD Rhapsody™ Whole Transcriptome Analysis Kit Workflow

In this video, we show you how to produce whole transcriptome analysis or WTA libraries using our BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit protocol.

Performing Quality Control on the Final Sequencing Libraries

In this video, we will demonstrate how to perform quality control on your final sequencing libraries using the Agilent Bioanalyzer.

BD Rhapsody™ Single-Cell Analysis System - Library Pooling

In this video, we will walk you through an example of pooling BD Rhapsody™ System libraries for sequencing. If you’re working with a sequencing facility or provider, make sure to discuss their requirements and recommendations prior to pooling your libraries.